Hemostasis

EDTA-Dependent Pseudothrombocytopenia in Blood Smear

Mechanism of EDTA-dependent pseudothrombocytopenia and in vitro platelet agglutination: a clinical example of spurious thrombocytopenia in blood smear microscopy.

Peripheral blood smear: large aggregate of agglutinated platelets
Peripheral blood smear: large aggregate of agglutinated platelets

Pseudothrombocytopenia

Pseudothrombocytopenia, caused by EDTA (ethylenediaminetetraacetic acid), is a phenomenon of in vitro platelet agglutination. This agglutination is mediated by autoantibodies that recognize platelet antigens modified by EDTA.

Clinical information: 39-year-old female, asymptomatic. Biological information: Blood test: no abnormalities, except for platelets 27 x 10^9/L. Sample type: Peripheral blood. Staining type: Hematoxylin-eosin. Authors: Montero Martín, Laura; López González, Sandra; Vera Guerrero, Elena; Sopeña Corvinos, María. Workplace: Hematology Department, Central Laboratory of the Community of Madrid, San Sebastián de los Reyes.