Laboratory methods

Shubich Method: Granulocyte Cationic Protein Detection

The principle of Shubich's cytochemical reaction with bromophenol blue: detecting cationic lysosomal proteins as granules in the cytoplasm of blood neutrophils.

Cytochemical reaction with bromophenol blue for cationic lysosomal proteins of granulocytes (Shubich method). Cationic proteins are detected as blue granules in the cytoplasm