Learn about the causes of dye crystal formation during Gram staining. This article covers errors in smear fixation technique and gentian violet crystallization on hot glass.
Flowers for March 8th. An error in Gram smear staining. The slide was fixed over a burner flame, and gentian violet was immediately poured onto the hot glass. As a result, we see altered dye structures resembling flowers or snowflakes in the preparation. (The students tried hard and gave us flowers on a slide 😆)
🙏 Photos kindly provided by Inna Storozheva, Clinical Diagnostic Laboratory of the Regional Hospital, Kaliningrad.
A clear example of gross violation of preparation rules for a flora smear test: spermatozoa found in a female sample. Preanalytical stage errors.
An analysis of an error in preparing Ziehl-Neelsen carbol fuchsin stain: why the dye solution unexpectedly turned green. A common lab mistake.
Learn the methodology and interpretation of eosin-nigrosin staining for sperm viability. Differentiate live from dead cells in semen analysis, crucial for diagnosing male infertility.
