Laboratory methods

Myelogram Cell Count: 300 or 500 Cells in Bone Marrow Smear

How many cells to count for a myelogram: comparing WHO recommendations for 500 cells and data on differentiating 300 bone marrow aspirate cells.

Bone marrow aspirate smear with polymorphic cellular composition
Bone marrow aspirate smear with polymorphic cellular composition

🔬🦴🩸MYELOGRAM. HOW MANY CELLS TO COUNT? ✅Counting 500 cells is recommended in the 2008 and 2017 WHO classifications and the 2008 ICSH guidelines, especially for assessing potential myeloid neoplasia. ✅Counting 300 cells in a bone marrow aspirate smear yields results statistically similar to those obtained from counting 500 cells for a myelogram. Such conclusions were published in 2018 in the American Journal of Clinical Pathology (link below). ✅Reducing the number of cells counted can lead to decreased labor costs without compromising the quality of medical care. Source: Abdulrahman A. A. et al. Is a 500-cell count necessary for bone marrow differentials? A proposed analytical method for validating a lower cutoff //American journal of clinical pathology. – 2018. – Т. 150. – №. 1. – С. 84-91.