SPERM REVITALIZATION SOLUTIONS (in vitro only!) If a large number of live spermatozoa are found in the ejaculate with a tendency towards significantly impaired motility, it is advisable to perform a test with “revitalizing” solutions. This is because temporary immobility of spermatozoa may have occurred during the transport of sperm (ejaculate). The following solutions can be used as “revitalizing” agents:
👉🏻Becker’s solution (glucose – 3 g, KH2PO4 – 0.6 g, NaCl – 0.2 g; KH2PO4 – 0.01 g, distilled water – up to 100 ml (pH 7.8); 👉🏻0.1% caffeine solution (ampoule caffeine should not be used); 0.1 M arginine solution; 👉🏻Ringer’s solution; 👉🏻Isotonic glucose solution.
❗Mix sperm with the revitalizing solution in a 1:10 ratio. Mix, and in a native preparation, after 5–10 minutes, immobile but live spermatozoa begin to move. The kinesiogram should be calculated in this preparation.
Source: Novikova I.A. - Introduction to Clinical Laboratory Diagnostics - 2018
