⚠Total antibodies to syphilis POSITIVE (+), but IgG NEGATIVE (-). How to interpret such test results? ❗Official answer from the developer of the VECTOR-BEST test system: “The RecombiBest anti-pallidum - IgG reagent kit detects class G immunoglobulins only to one antigen, the so-called TmpA (or otherwise, a protein with a molecular weight of 42-45 kD). The body of a patient infected with Treponema pallidum primarily begins to synthesize specific antibodies to this antigen, and these same antibodies are eliminated from the patient’s body earlier than others after successful treatment, which makes it possible to monitor the patient dynamically (by determining the titer of antibodies to TmpA with the RecombiBest anti-pallidum - IgG kit). In the presence of active syphilitic infection in the examined patient, the sensitivity of the RecombiBest anti-pallidum - IgG kit is the same as that of enzyme immunoassay kits used for screening. In cases where residual antibodies to other antigens persist in the patient’s body after effectively conducted treatment, the aforementioned reagent kit does not detect them. Patients who have obtained positive results in the screening test for total antibodies and negative results in tests for immunoglobulins G (RecombiBest anti-pallidum - IgG) and immunoglobulins (RecombiBest anti-pallidum - IgM) most likely had syphilis previously (syphilis in anamnesis). Since there are no reagent kits in real life that have 100% sensitivity and specificity, there is, of course, some probability that a false positive result (FPR) was obtained in the screening test. To differentiate between FPR and syphilis in anamnesis, it is necessary to analyze such samples in another specific treponemal test with sensitivity and specificity close to ELISA, for example, in TPPA.” ✅Attached are 3 PDF files - ELISA instructions for syphilis. 💎More than 60 instructions for ELISA kits can be found in our LAB-TESTS library.
👉The post uses a photograph of Henri Gervex’s painting - Rolla.
