https://www.leukoformula.com/en/tags/gemosiderin/Recent content in Hemosiderin on LeukoformulaHugoen-USTue, 10 Jun 2025 08:02:25 +0300https://www.leukoformula.com/en/post/gemosiderin-v-osadke-mochi-mikroskopiya-i-10737/Tue, 10 Jun 2025 08:02:25 +0300https://www.leukoformula.com/en/post/gemosiderin-v-osadke-mochi-mikroskopiya-i-10737/<p>Microscopy of urine sediment. Hemosiderin.</p> <p>The presence of hemosiderin in urine is a result of blood cell destruction, where hemoglobin is released from erythrocytes into the bloodstream beyond the binding capacity of haptoglobin. Proximal tubular cells are damaged, slough off along with hemosiderin, and are excreted in the urine, giving it a brownish color. Hemosiderinuria is usually observed three to four days after the onset of hemolytic conditions. Hemosiderin can remain in the urine for several weeks. Hemosiderinuria is a better marker of intravascular hemolysis than hemoglobinuria. Hemosiderin is not observed in the urine of healthy individuals. Hemosiderin is readily soluble in acids. Perls&rsquo; reaction, or the Prussian blue reaction, is specific for hemosiderin.</p>https://www.leukoformula.com/en/post/reakciya-perlsa-v-mokrote-kletki-serdechnyh-5893/Mon, 30 Dec 2024 14:30:00 +0300https://www.leukoformula.com/en/post/reakciya-perlsa-v-mokrote-kletki-serdechnyh-5893/<p>Positive Perls&rsquo; reaction in alveolar macrophages. Perls&rsquo; reaction is a method for detecting trivalent iron within hemosiderin and ferritin. The reagents used are potassium ferrocyanide and hydrochloric acid of equal concentration (5%) and equal volume. Hydrochloric acid ensures the availability of the iron cation by breaking down hemosiderin and forming iron(III) chloride. Iron chloride, in turn, reacts with the potassium ferrocyanide solution, forming a mixture of hexacyanoferrates(II) – pigments collectively known as Prussian blue. In laboratory diagnostics and histology, this reaction is used to detect hemosiderin in macrophages. Alveolar macrophages laden with hemosiderin are called &ldquo;heart failure cells&rdquo;. Such cells originate from alveolar septal macrophages that have engulfed erythrocyte debris, with hemoglobin being converted into hemosiderin. Heart failure cells accumulate in the alveolar lumen during pulmonary congestion. Sputum smear, stained with Perls&rsquo; reaction. Magnification x100, x200, x400</p>